FTL Antikörper (AA 38-165)

Produktnummer ABIN3025210

Dieses Anti-FTL-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von FTL in WB, IF, FACS und IHC (p). Geeignet für Human.

Kurzübersicht für FTL Antikörper (AA 38-165) (ABIN3025210)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: FTLC1-1

Produktdetails anti-FTL Antikörper

Bindungsspezifität: AA 38-165

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. Western blot: 1-2 μg/mL,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Ferritin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Andere Bezeichnung: Ferritin (Light Chain Specific)

Hintergrund: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Gen-ID: 2512

Pathways: Transition Metal Ion Homeostasis