MUC1 Antikörper

Produktnummer ABIN3025208

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MUC1 in WB, FACS, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MUC1 Antikörper (ABIN3025208)

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: VU-2G7

Produktdetails anti-MUC1 Antikörper

Produktmerkmale: mAb VU-2G7 reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with "epitope fingerprinting". The extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Aufreinigung: Protein G affinity chromatography

Immunogen: A synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3- COOH was used as the immunogen for the MUC-1 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MUC-1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (Formalin-paraffin): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the MUC-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC-1

Hintergrund: MAb VU-2G7 reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with "epitope fingerprinting". The extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

Pathways: Negative Regulation of intrinsic apoptotic Signaling