Biotin Antikörper

Produktnummer ABIN3025157

Dieser Maus Monoklonal Antikörper detektiert spezifisch Biotin in WB, FACS, IF, IHC (p), CM und ISH.

Kurzübersicht für Biotin Antikörper (ABIN3025157)

Target: Biotin

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Biotin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Confocal Microscopy (CM), In situ hybridization (ISH)

Klon: SPM375

Produktdetails anti-Biotin Antikörper

Produktmerkmale: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Aufreinigung: Protein G affinity chromatography

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.\. Confocal Microscopy,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,In situ Nucleic Acid Hybridization,Western: blot 1-3 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.