Biotin Antikörper

Produktnummer ABIN3025157

Dieser Maus Monoklonal Antikörper detektiert spezifisch Biotin in WB, FACS, IF und IHC (p).

Kurzübersicht für Biotin Antikörper (ABIN3025157)

Target: Biotin

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Biotin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: SPM375

Produktdetails anti-Biotin Antikörper

Verwendungszweck: Anti-Biotin Antibody (azide and preservative free)

Produktmerkmale: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Aufreinigung: Protein G affinity chromatography

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Biotin

Target: Biotin

Substanzklasse: Chemical

Hintergrund: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.