FLI1 Antikörper (AA 107-216)

Produktnummer ABIN3024862

Produktdetails anti-FLI1 Antikörper

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Bindungsspezifität: AA 107-216

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FLI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Aufreinigung: Protein G affinity chromatography

Immunogen: Human partial recombinant protein (amino acids 107-216) was used as the immunogen for this FLI1 antibody.

Klon: FLVI1-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FLI1 antibody to be titered up or down for optimal performance.\. Western blot: 1-2 μg/m,l,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the FLI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Andere Bezeichnung: FLI1 (FLI1 Produkte)

Hintergrund: Friend leukemia integration 1 is a member of the ETS family of DNA binding transcription factors that is involved in cellular proliferation and tumorigenesis. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Gen-ID: 2313