Fibronectin Antikörper

Produktnummer ABIN3024852

Der Maus Monoklonal anti-Fibronectin Antikörper wird verwendet zum Nachweis von Fibronectin in Proben von Human. Er wurde validiert für IF, FACS und IHC (p).

Kurzübersicht für Fibronectin Antikörper (ABIN3024852)

Target: Fibronectin

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: TV-1

Produktdetails anti-Fibronectin Antikörper

Verwendungszweck: Fibronectin Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: A T-cell lymphoma biopsy was used as the immunogen for this fibronectin antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Fibronectin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectin is a soluble dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. There are two types of fibronectin: soluble fibronectin which is produced by hepatocytes and found in blood plasma, and insoluble cellular fibronectin which is produced by fibroblasts in a soluble form and latter assembled into an insoluble form via fibril formation.

This antibody reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this antibody localizes to tumor vessels where it binds to the underlying basement. The Fibronectin epitope recognized by this antibody is not accessible in normal tissues to the circulating antibody indicating that it can be used to specifically target tumor vessels in vivo. Clone TV-1 fibronectin antibody is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or antibodies.