ESR2 Antikörper (C-Term)

Produktnummer ABIN3024797

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ESR2 in WB, IF, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schaf, Schwein, Pferd und Affe.

Kurzübersicht für ESR2 Antikörper (C-Term) (ABIN3024797)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human, Maus, Ratte, Schaf, Schwein, Pferd, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: ERb455

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Protein G purified monoclonal antibody

Immunogen: The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Estrogen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: Estrogen Receptor beta

Hintergrund: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Gen-ID: 2100

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling