Elastase 3B Antikörper (AA 82-238)

Produktnummer ABIN3024740

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Elastase 3B in ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Elastase 3B Antikörper (AA 82-238) (ABIN3024740)

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Elastase 3B Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: ELTS3B-2

Produktdetails anti-Elastase 3B Antikörper

Bindungsspezifität: AA 82-238

Aufreinigung: Protein G

Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: 2-4 μg/mL, order BSA free format for coating,IF: 1-2 μg/mL, IHC-P: 1-2 μg/mL (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Andere Bezeichnung: Elastase 3B

Hintergrund: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.