EpCAM Antikörper (Cytoplasmic Domain)

Produktnummer ABIN3024723

Der Maus Monoklonal anti-EpCAM Antikörper wird verwendet zum Nachweis von EpCAM in Proben von Human, Maus, Ratte, Cat und Hund. Er wurde validiert für IHC (p).

Kurzübersicht für EpCAM Antikörper (Cytoplasmic Domain) (ABIN3024723)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human, Maus, Ratte, Cat, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: EGP40-1110

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: AA 289-314, Cytoplasmic Domain

Verwendungszweck: EpCAM Antibody / Cytoplasmic domain

Aufreinigung: Protein G affinity chromatography

Immunogen: A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the EpCAM antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: EpCAM

Hintergrund: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

UniProt: P16422