FBXO5 Antikörper (AA 1-250)

Produktnummer ABIN3024715

Dieses Anti-FBXO5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von FBXO5 in WB, IF, FACS und IHC (p). Geeignet für Human.

Kurzübersicht für FBXO5 Antikörper (AA 1-250) (ABIN3024715)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FBXO5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: EMI1-1176

Produktdetails anti-FBXO5 Antikörper

Bindungsspezifität: AA 1-250

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant fragment (203 amino acid residues between aa 1-250) from the human protein was used as the immunogen for the EMI1 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the EMI1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the EMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Andere Bezeichnung: EMI1

Hintergrund: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Pathways: Mitotic G1-G1/S Phases