FBXO5 Antikörper (N-Term)

Produktnummer ABIN3024714

Der Maus Monoklonal anti-FBXO5 Antikörper wird verwendet zum Nachweis von FBXO5 in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für FBXO5 Antikörper (N-Term) (ABIN3024714)

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FBXO5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: FBXP5

Produktdetails anti-FBXO5 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: EMI-1 Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the EMI-1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the EMI-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Andere Bezeichnung: EMI-1

Hintergrund: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases