FBXO5 Antikörper (N-Term)

Produktnummer ABIN3024714

Produktdetails anti-FBXO5 Antikörper

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FBXO5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Klon: FBXP5

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the EMI-1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the EMI-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Andere Bezeichnung: EMI-1 (FBXO5 Produkte)

Hintergrund: Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Pathways: Mitotic G1-G1/S Phases