Cytokeratin 8/18 Antikörper

Produktnummer ABIN3024678

Der Maus Monoklonal anti-Cytokeratin 8/18 Antikörper wird verwendet zum Nachweis von Cytokeratin 8/18 in Proben von Human. Er wurde validiert für IF, WB, FACS und IHC (p).

Kurzübersicht für Cytokeratin 8/18 Antikörper (ABIN3024678)

Target: Cytokeratin 8/18

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 8/18 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: C-51

Produktdetails anti-Cytokeratin 8/18 Antikörper

Verwendungszweck: Cytokeratin 8/18 Antibody (azide and preservative free)

Keine Kreuzreaktivität: Maus, Ratte (Rattus), Frettchen, Kaninchen, Esel, Huhn, Xenopus laevis

Aufreinigung: Protein G affinity chromatography

Immunogen: A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8/18 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cytokeratin 8/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Cytokeratin 8/18

Target: Cytokeratin 8/18

Hintergrund: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

UniProt: P05787