Cytokeratin 8/18 Antikörper

Produktnummer ABIN3024676

Dieses Anti-Cytokeratin 8/18-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytokeratin 8/18 in WB, IF, FACS und IHC (p). Geeignet für Human, Rind (Kuh), Affe, Schwein und Schaf.

Kurzübersicht für Cytokeratin 8/18 Antikörper (ABIN3024676)

Target: Cytokeratin 8/18

Reaktivität: Human, Rind (Kuh), Affe, Schwein, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 8/18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: C-51

Produktdetails anti-Cytokeratin 8/18 Antikörper

Keine Kreuzreaktivität: Maus, Ratte (Rattus), Frettchen, Kaninchen, Esel, Huhn, Xenopus laevis

Aufreinigung: Protein G affinity chromatography

Immunogen: A cytoskeleton preparation from HeLa cells was used as the immunogen for the Cytokeratin 8 + 18 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Cytokeratin 8 + 18 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cytokeratin 8 + 18 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cytokeratin 8/18

Target: Cytokeratin 8/18

Andere Bezeichnung: Cytokeratin 8 + 18

Hintergrund: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.