KRT14 Antikörper (C-Term)

Produktnummer ABIN3024621

Der Maus Monoklonal Anti-KRT14-Antikörper wurde für WB, IF, FACS und IHC (p) validiert. Er ist geeignet, KRT14 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für KRT14 Antikörper (C-Term) (ABIN3024621)

Target: KRT14 (Keratin 14 (KRT14))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: LL002

Produktdetails anti-KRT14 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Protein G affinity chromatography

Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14 was used as the immunogen.

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cytokeratin 14 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu KRT14

Target: KRT14 (Keratin 14 (KRT14))

Andere Bezeichnung: Cytokeratin 14

Hintergrund: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). Cytokeratin 14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. antibody to cytokeratin 14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. cytokeratin 14 antibody is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Cytokeratin 14 antibody is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Gen-ID: 3861