Cytokeratin 19 Antikörper (AA 312-335)

Produktnummer ABIN3024555

Der Maus Monoklonal Anti-Cytokeratin 19 Antikörper wurde für WB, FACS, IF und IHC (p) validiert. Er ist geeignet, Cytokeratin 19 in Proben von Human zu detektieren.

Kurzübersicht für Cytokeratin 19 Antikörper (AA 312-335) (ABIN3024555)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM266

Produktdetails anti-Cytokeratin 19 Antikörper

Bindungsspezifität: AA 312-335

Aufreinigung: Protein G affinity chromatography

Immunogen: The human breast cancer cell line MCF-7 was used as the immunogen for this anti-CK19 antibody. Its epitope maps between amino acids 312-335.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-CK19 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-CK19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Andere Bezeichnung: CK19

Hintergrund: CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast.