CD99 Antikörper

Produktnummer ABIN3024516

Der Maus Monoklonal anti-CD99 Antikörper wird verwendet zum Nachweis von CD99 in Proben von Human und Ratte. Er wurde validiert für WB, FACS, IF und IHC (p).

Kurzübersicht für CD99 Antikörper (ABIN3024516)

Target: CD99

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: HO36-1-1

Produktdetails anti-CD99 Antikörper

Verwendungszweck: CD99 Antibody (azide and preservative free)

Aufreinigung: PEG precipitation

Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes were used as the immunogen for the CD99 antibody.

Isotyp: IgM, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD99 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99

Hintergrund: CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. [UniProt]

UniProt: P14209