CD45RB Antikörper

Produktnummer ABIN3024427

Dieses Anti-CD45RB-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD45RB in FACS, IF, WB und IHC (p). Geeignet für Human.

Kurzübersicht für CD45RB Antikörper (ABIN3024427)

Target: CD45RB

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45RB Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PD7-26

Produktdetails anti-CD45RB Antikörper

Aufreinigung: Protein G purified

Immunogen: Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD45RB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD45RB

Target: CD45RB

Andere Bezeichnung: CD45RB

Hintergrund: CD45RB is one of the four major isoforms of CD45.  The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved.  Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms (< a href=../cd45-antibody-cocktail-2b11-pd726-v2243>Cat No V2243).

Gen-ID: 5788