CA 19-9 Antikörper

Produktnummer ABIN3024244

Dieses Anti-CA 19-9-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CA 19-9 in FACS, IF und IHC (p). Geeignet für Human.

Kurzübersicht für CA 19-9 Antikörper (ABIN3024244)

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA 19-9 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 121SLE

Produktdetails anti-CA 19-9 Antikörper

Aufreinigung: PEG precipitation

Immunogen: Precipitin lines obtained after immuno-diffusion using mAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le) were used as the immunogen for the CA19-9 antibody.

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CA19-9 antibody should be determined by the researcher.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CA19-9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CA 19-9

Target: CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Andere Bezeichnung: CA19-9

Hintergrund: CA19-9, a carbohydrate epitope expressed on a high MW (>400  kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This mAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.