CERK Antikörper (Extracellular Domain, N-Term)

Produktnummer ABIN302420

Der Kaninchen Polyklonal anti-CERK Antikörper wird verwendet zum Nachweis von CERK in Proben von Human. Er wurde validiert für IHC (p).

Kurzübersicht für CERK Antikörper (Extracellular Domain, N-Term) (ABIN302420)

Target: CERK (Ceramide Kinase (CERK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CERK Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CERK Antikörper

Bindungsspezifität: Extracellular Domain, N-Term

Spezifität: This antibody detects Ceramide Kinase (CERK), N-terminal extracellular domain.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: CERK antibody was raised against synthetic peptide from N-terminal extracellular domain of human CERK.

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on paraffin sections (20 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % sodium azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Details zu CERK

Target: CERK (Ceramide Kinase (CERK))

Andere Bezeichnung: Ceramide Kinase

Hintergrund: CERK, which phosphorylates Cer to produce ceramide 1-phosphate (C1P), was initially described as a Ca2+-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in polymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis.Synonyms: Acylsphingosine kinase, KIAA1646, LK4, Lipid kinase 4, hCERK

Gen-ID: 64781

NCBI Accession: NP_073603

UniProt: Q8TCT0