CD147 Antikörper (Extracellular Domain)

Produktnummer ABIN3024195

Der Maus Monoklonal Anti-CD147-Antikörper wurde für FACS, IF und IHC (p) validiert. Er ist geeignet, CD147 in Proben von Human zu detektieren.

Kurzübersicht für CD147 Antikörper (Extracellular Domain) (ABIN3024195)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD147 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CDLA147

Produktdetails anti-CD147 Antikörper

Bindungsspezifität: Extracellular Domain

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the CD147 antibody. The epitope is known to be extracellular.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Titering of the CD147 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the CD147 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Andere Bezeichnung: CD147

Hintergrund: Basigin (BSG) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147) is a protein that in humans is encoded by the BSG gene. This protein is a determinant for the Ok blood group system. Basigin has been shown to be an essential receptor on red blood cells for the human malaria parasite, Plasmodium falciparum. It has a variety of functions. In addition to its metalloproteinase-inducing ability, basigin also regulates several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. CD147 is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes. [Wiki]

Pathways: S100 Proteine