Melanoma Antikörper

Produktnummer ABIN3024167

Dieser Maus Monoklonal Antikörper erkennt spezifisch Melanoma in IHC (p) und IF. Er zeigt eine Reaktivität gegenüber Human, Hund und Maus.

Kurzübersicht für Melanoma Antikörper (ABIN3024167)

Target: Melanoma

Reaktivität: Human, Hund, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Klon: PNL2

Produktdetails anti-Melanoma Antikörper

Produktmerkmale: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.

Reinheit: Protein G affinity chromatography

Immunogen: Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mg/mL in sterile PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store the Melanoma antibody PNL2 at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Melanoma

Target: Melanoma