Rekombinanter WT1 Antikörper

Produktnummer ABIN3024065

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch WT1 in IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter WT1 Antikörper (ABIN3024065)

Target: WT1 (Wilms Tumor 1 (WT1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RMWT1-1

Produktdetails anti-WT1 Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: A recombinant protein was used as the immunogen for the WT1 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the WT1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the WT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1

Hintergrund: Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Pathways: Tube Formation