pan Keratin Antikörper

Produktnummer ABIN3024003

Der Maus Monoklonal Anti-pan Keratin-Antikörper wurde für IF, FACS, WB und IHC (p) validiert. Er ist geeignet, pan Keratin in Proben von Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen und Huhn zu detektieren.

Kurzübersicht für pan Keratin Antikörper (ABIN3024003)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PAN-CK (Cocktail)

Produktdetails anti-pan Keratin Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Human epidermal keratin was used as the immunogen for the pan Cytokeratin antibody cocktail.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the pan Cytokeratin antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the pan Cytokeratin antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Pan Cytokeratin

Hintergrund: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, with 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 55  kDa (CK7), 52  kDa (CK8), 56.5  kDa (CK10), 53  kDa (CK13), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 46  kDa (CK17), 45  kDa (CK18) and 40  kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRT-PAN is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It is useful in characterizing the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and shows high sensitivity in the recognition of epithelial cells and carcinomas.