pan Keratin Antikörper (PE)

Produktnummer ABIN3023996

Dieses Maus Monoklonal-Antikörper erkennt spezifisch pan Keratin in IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen und Huhn.

Kurzübersicht für pan Keratin Antikörper (PE) (ABIN3023996)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit PE

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: AE1-AE3

Produktdetails anti-pan Keratin Antikörper (PE)

Reinheit: Protein G affinity chromatography

Immunogen: Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.\. Flow Cytometry : 5 μL/test/million cells or 5 μL/test/100 μL of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: 500 μL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the pan Cytokeratin antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Pan Cytokeratin

Hintergrund: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.