LAMP3 Antikörper (full length)

Produktnummer ABIN3023872

Der Maus Monoklonal anti-LAMP3 Antikörper (Klon LAMP3-968) (ABIN3023872) detektiert spezifisch LAMP3 in IHC (p) und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für LAMP3 Antikörper (full length) (ABIN3023872)

Target: LAMP3 (Lysosomal-Associated Membrane Protein 3 (LAMP3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAMP3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Klon: LAMP3-968

Produktdetails anti-LAMP3 Antikörper

Bindungsspezifität: full length

Verwendungszweck: LAMP-3 Antibody / CD63 (azide and preservative free)

Produktmerkmale: This mAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63/LAMP3. Its epitope is different from that of mAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63/LAMP3 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human full-length protein was used as the immunogen for the CD63 / LAMP-3 antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LAMP-3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the LAMP-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu LAMP3

Target: LAMP3 (Lysosomal-Associated Membrane Protein 3 (LAMP3))

Andere Bezeichnung: LAMP-3

Hintergrund: This mAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63/LAMP-3. Its epitope is different from that of mAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63/LAMP-3 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

UniProt: P08962