Keratin 10 Antikörper

Produktnummer ABIN3023828

Produktdetails anti-Keratin 10 Antikörper

Target: Keratin 10 (KRT10)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 10 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: This mAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.

Klon: KRT10-844

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Keratin 10 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Keratin 10 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Keratin 10

Target: Keratin 10 (KRT10)

Abstract: KRT10 Produkte

Synonyme: BCIE antikoerper, BIE antikoerper, CK10 antikoerper, EHK antikoerper, K10 antikoerper, KPP antikoerper, D130054E02Rik antikoerper, K1C1 antikoerper, Krt-1.10 antikoerper, Krt1-10 antikoerper, Ka10 antikoerper, Ker10 antikoerper, KRT10 antikoerper, KRT25 antikoerper, KRT27 antikoerper, KRT28 antikoerper, keratin 10 antikoerper, keratin 10, type I S homeolog antikoerper, keratin, type I cytoskeletal 25 antikoerper, KRT10 antikoerper, Krt10 antikoerper, krt10.S antikoerper, LOC101085587 antikoerper

Hintergrund: This mAb recognizes a protein of 56.5  kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.