ICAM1 Antikörper

Produktnummer ABIN302240

Der Maus Monoklonal anti-ICAM1 Antikörper wird verwendet zum Nachweis von ICAM1 in Proben von Human. Er wurde validiert für WB, FACS, IF, IHC (p) und IHC (fro).

Kurzübersicht für ICAM1 Antikörper (ABIN302240)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 1H4

Produktdetails anti-ICAM1 Antikörper

Produktmerkmale: Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

Aufreinigung: Protein A Affinity Chromatography

Immunogen: Raji cells and spleen cells fused with NS1 cells

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Western blot. Immunohistochemistry (paraffin sections): 10-20 μg/mL (Prolonged fixation in bufferedformalin can destroy the epitope, hight temperature antigen unmasking technique isrequired). Immunohistochemistry (frozen sections): 5-20 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Azide free PBS preserved by filter sterilization, pH ~7.4

Konservierungsmittel: Azide free

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Andere Bezeichnung: CD54 / ICAM1

Hintergrund: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling