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Keratin 10/13 Antikörper

Der Maus Monoklonal Anti--Antikörper wurde für WB, IHC (p) und IHC (fro) validiert. Er ist geeignet, in Proben von Human und Cat zu detektieren. Es sind 3+ Publikationen verfügbar.
Produktnummer ABIN302093

Kurzübersicht für Keratin 10/13 Antikörper (ABIN302093)

Target

Keratin 10/13

Reaktivität

  • 13
  • 12
  • 1
Human, Cat

Wirt

  • 13
Maus

Klonalität

  • 13
Monoklonal

Konjugat

  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Unkonjugiert

Applikation

  • 12
  • 12
  • 10
  • 5
  • 4
  • 3
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

DE-K13
  • Verwendungszweck

    Anti-Cytokeratin 10/13 Purified

    Spezifität

    The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

    Kreuzreaktivität (Details)

    Human, Feline (Cat), Other species Not tested

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Cytoskeletal preparation extracted from human ectocervical epithelium.

    Isotyp

    IgG2a
  • Applikationshinweise

    Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/mL (1:100), positive tissue: tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH . The antibody DE-K13 reacts exclusively with Cytokeratin 13.
    Immunohistochemistry (frozen sections): Use at an assay dependent dilution.
    Western blotting: recommended dilution: 1-3 μg/mL.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Kozaki, Nakamura, Iguchi, Kano, Watanabe, Fujiwara, Hasegawa: "Immunohistochemical analysis of cytokeratin expression in dog skin." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 1, pp. 1-4, (2001) (PubMed).

    Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard, Misdorp: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." in: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).

    Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).

  • Target

    Keratin 10/13

    Andere Bezeichnung

    Cytokeratin 10/13

    Hintergrund

    Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).,K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13
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