CD1a Antikörper (APC)

Produktnummer ABIN301984

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD1a in FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 9+ Publikationen erwähnt.

Kurzübersicht für CD1a Antikörper (APC) (ABIN301984)

Target: CD1a

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD1a Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: HI149

Produktdetails anti-CD1a Antikörper (APC)

Verwendungszweck: Anti-Hu CD1a APC

Spezifität: The antibody HI149 reacts with an extracellular epitope of CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas. The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.

Kreuzreaktivität (Details): Other not tested, Human

Aufreinigung: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human thymocytes

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.
Avoid prolonged exposure to light.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD1a Antikörper (APC) (ABIN301984)

Mayer, Irschick, Moser, Wurm, Huemer, Romani, Irschick: "Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers." in: Investigative ophthalmology & visual science, Vol. 48, Issue 10, pp. 4459-67, (2007) (PubMed).

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, (2007) (PubMed).

Perros, Dorfmüller, Souza, Durand-Gasselin, Mussot, Mazmanian, Hervé, Emilie, Simonneau, Humbert: "Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension." in: The European respiratory journal, Vol. 29, Issue 3, pp. 462-8, (2007) (PubMed).

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, (2005) (PubMed).

Chen, Murakami, Oppenheim, Howard: "Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking." in: Blood, Vol. 106, Issue 7, pp. 2409-16, (2005) (PubMed).

Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, (2005) (PubMed).

Hunger, Sieling, Ochoa, Sugaya, Burdick, Rea, Brennan, Belisle, Blauvelt, Porcelli, Modlin: "Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells." in: The Journal of clinical investigation, Vol. 113, Issue 5, pp. 701-8, (2004) (PubMed).

McKenna, Asplund, Kroft: "Immunophenotypic analysis of hematogones (B-lymphocyte precursors) and neoplastic lymphoblasts by 4-color flow cytometry." in: Leukemia & lymphoma, Vol. 45, Issue 2, pp. 277-85, (2004) (PubMed).

Säemann, Parolini, Böhmig, Kelemen, Krieger, Neumüller, Knarr, Kammlander, Hörl, Diakos, Stuhlmeier, Zlabinger: "Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells." in: Journal of leukocyte biology, Vol. 71, Issue 2, pp. 238-46, (2002) (PubMed).

Details zu CD1a

Target: CD1a

Andere Bezeichnung: CD1a

Hintergrund: CD1a molecule,CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.,T6, Leu-6, HTA1, FCB6

Gen-ID: 909

UniProt: P06126

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process