Tumor Protein p73 Antikörper (N-Term)

Produktnummer ABIN298091

Der Maus Monoklonal Anti-Tumor Protein p73 Antikörper wurde für WB, IHC, IHC (p), IP und ICC validiert. Er ist geeignet, Tumor Protein p73 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Tumor Protein p73 Antikörper (N-Term) (ABIN298091)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Tumor Protein p73 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 5B429

Produktdetails anti-Tumor Protein p73 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

Aufreinigung: Protein G purified

Immunogen: Full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), IP, WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS containing 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Andere Bezeichnung: TP73 / p73

Hintergrund: Name/Gene ID: TP73

Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

Gen-ID: 7161

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus