Neural Wiskott-Aldrich syndrome protein (WASL) (AA 21-270) Antikörper

Produktnummer ABIN297874

Produktdetails

Target: Neural Wiskott-Aldrich syndrome protein (WASL)

Bindungsspezifität: AA 21-270

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Spezifität: Selected for its ability to recognize human N-WASP in the applications listed.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rhN-WASP (aa21-270).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 1.0 μg/mL with the appropriate secondary reagents to detect N-WASP. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human N-WASP on A431 human epidermoid carcinoma cells stimulated with EGF. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human N-WASP. The detection limit for rhN-WASP is approximately 1.0 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Antigendetails

Target: Neural Wiskott-Aldrich syndrome protein (WASL)

Andere Bezeichnung: WASL / N-WASP (WASL Produkte)

Hintergrund: Name/Gene ID: WASL

Synonyms: WASL, N-WASP, NWASP, Wiskott-Aldrich syndrome-like

Gen-ID: 8976

UniProt: O00401