SOX15 Antikörper

Produktnummer ABIN297700

Produktdetails anti-SOX15 Antikörper

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser SOX15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Spezifität: Selected for its ability to recognize human SOX15 in the applications listed.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rhSOX15.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 20 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 1 % cross-reactivity with rhSOX1, -2, -5, -6, -7, -10, -12, -14, -15, -17, and rhSOX21. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect SOX15 in NTera2 teratocarcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human SOX15. The detection limit for rhSOX15 is approximately 0.3 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu SOX15

Target: SOX15 (SRY (Sex Determining Region Y)-Box 15 (SOX15))

Andere Bezeichnung: SOX15 (SOX15 Produkte)

Hintergrund: Name/Gene ID: SOX15

Synonyms: SOX15, Protein SOX-15, Protein SOX-20, SOX26, SOX20, Protein SOX-12, SOX27

Gen-ID: 6665

UniProt: O60248