Lipin 1 Antikörper (AA 448-574)

Produktnummer ABIN297253

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Lipin 1 in WB, ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Lipin 1 Antikörper (AA 448-574) (ABIN297253)

Target: Lipin 1 (LPIN1)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Lipin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-Lipin 1 Antikörper

Bindungsspezifität: AA 448-574

Spezifität: Selected for its ability to recognize human Lipin 1 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 2 % cross-reactivity with rhLipin2 and rhLipin3.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rhLipin 1 (aa448-574).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, WB

Usage: Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect human Lipin 1 on human MSC-derived adipocytes. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 2 ng/lane under non-reducing and reducing conditions. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Lipin 1. The detection limit for rhLipin 1 is approximately 0.1 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu Lipin 1

Target: Lipin 1 (LPIN1)

Andere Bezeichnung: LPIN1 / Lipin 1

Hintergrund: Name/Gene ID: LPIN1

Synonyms: LPIN1, Lipin-1, Lipin 1, PAP1, KIAA0188

Gen-ID: 23175

UniProt: Q14693

Pathways: Monocarboxylic Acid Catabolic Process