HAPLN4 Antikörper

Produktnummer ABIN297170

Der Ziege-Polyklonal Anti-HAPLN4-Antikörper wurde für WB, ICC und IP validiert. Er ist geeignet zum Nachweis von HAPLN4 in Proben aus Human. }

Kurzübersicht für HAPLN4 Antikörper (ABIN297170)

Target: HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HAPLN4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Produktdetails anti-HAPLN4 Antikörper

Spezifität: Selected for its ability to recognize human HAPLN4 in the applications listed. In Western Blots, this antibody shows less than 1 % cross-reactivity with rhHAPLN1.

Aufreinigung: Affinity purified

Immunogen: NS0-derived rhHAPLN4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IP, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HAPLN4. The detection limit for rhHAPLN4 is approximately 10 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhHAPLN4 from conditioned media of transfected NS0 cells. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HAPLN4 on SHSY5Y human neuroblastoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights 557-coupled donkey anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu HAPLN4

Target: HAPLN4 (Hyaluronan and Proteoglycan Link Protein 4 (HAPLN4))

Andere Bezeichnung: HAPLN4

Hintergrund: Name/Gene ID: HAPLN4

Synonyms: HAPLN4, BRAL2, KIAA1926, Brain link protein 2

Gen-ID: 404037

UniProt: Q86UW8