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CD99 Antikörper (Extracellular Domain)

CD99 Reaktivität: Human FACS, WB, ELISA Wirt: Ziege Polyclonal unconjugated

Produktnummer ABIN296822

Datenblatt (PDF)

Target Alle CD99 Antikörper anzeigen

CD99
Bindungsspezifität
15

6

4

4

3

2

2

2

1

1

1

1

1

1
Extracellular Domain
Reaktivität
102

24

9

6

1
Human
Wirt
73

50

3
Ziege
Passende Sekundärantikörper
Klonalität
72

54
Polyklonal
Konjugat
49

12

11

8

4

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD99 Antikörper ist unkonjugiert
Applikation
62

52

36

29

21

18

14

13

13

12

6

5

4

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

Spezifität

Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmCD99.

Aufreinigung

Affinity purified

Immunogen

NS0-derived rhCD99 extracellular domain.

Isotyp

IgG

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CD99 Reaktivität: Ratte WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

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Applikationshinweise

Approved: ELISA, Flo, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well.

Kommentare

Target Species of Antibody: Human

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitute in 500 μL of Sterile PBS.

Konzentration

Lot specific

Buffer

Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.

Handhabung

Avoid repeat freeze-thaw cycles.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20°C. Avoid freeze-thaw cycles.
Target

CD99

Andere Bezeichnung

CD99 (CD99 Produkte)

Substanzklasse

Viral Protein

Hintergrund

Name/Gene ID: CD99

Synonyms: CD99, 12E7, CD99 antigen, MIC2X, MIC2, Surface antigen MIC2, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2, CD99 Molecule, E2 antigen, HBA71

Gen-ID

4267