FHL1 Antikörper

Produktnummer ABIN7306298

Dieser unabhängig validierte Kaninchen Polyklonal Antikörper detektiert spezifisch FHL1 in WB, IF und IC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für FHL1 Antikörper (ABIN7306298)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-FHL1 Antikörper

Spezifität: Recognizes endogenous levels of FHL-1 protein.

Produktmerkmale: Rabbit polyclonal antibody to FHL-1

Aufreinigung: The antibody was purified by immunogen affinity chromatography.

Immunogen: Recombinant full length protein of human FHL-1

Anwendungsinformationen

Applikationshinweise: WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #101371 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101371

Datum: 19.07.2017

Antigen: FHL-1

Chargennummer: AL1F04A

Validierte Anwendung: Immunofluorescence

Positivkontrolle: MRL/lpr lupus mice (14 weeks of age)

Negativkontrolle: FH knockout MRL/lpr lupus mice

Bewertung: Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.

Validierungsbilder

IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Protokoll

Primärantikörper: ABIN2966633

Sekundärantikörper: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Handhabung

Format: Liquid

Buffer: Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Andere Bezeichnung: FHL-1

Hintergrund: SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1

Gen-ID: 2273, 14199, 25177

UniProt: Q13642, P97447, Q9WUH4