FHL1 Antikörper

Produktnummer ABIN7306298

Dieses unabhängig validierte Kaninchen Polyklonal-Antikörper erkennt spezifisch FHL1 in WB, IF und IC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für FHL1 Antikörper (ABIN7306298)

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FHL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-FHL1 Antikörper

Spezifität: Recognizes endogenous levels of FHL-1 protein.

Produktmerkmale: Rabbit polyclonal antibody to FHL-1

Aufreinigung: The antibody was purified by immunogen affinity chromatography.

Immunogen: Recombinant full length protein of human FHL-1

Anwendungsinformationen

Applikationshinweise: WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #101371 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101371

Datum: 19.07.2017

Antigen: FHL-1

Chargennummer: AL1F04A

Validierte Anwendung: Immunofluorescence

Positivkontrolle: MRL/lpr lupus mice (14 weeks of age)

Negativkontrolle: FH knockout MRL/lpr lupus mice

Bewertung: Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.

Validierungsbilder

IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Protokoll

Primärantikörper: ABIN2966633

Sekundärantikörper: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Handhabung

Format: Liquid

Buffer: Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Andere Bezeichnung: FHL-1

Hintergrund: SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1

Gen-ID: 2273, 14199, 25177

UniProt: Q13642, P97447, Q9WUH4