CCL5 Antikörper

Produktnummer ABIN296462

Der Schaf Polyklonal Anti-CCL5-Antikörper wurde für WB, ELISA, Neut und Func validiert. Er ist geeignet, CCL5 in Proben von Cat zu detektieren.

Kurzübersicht für CCL5 Antikörper (ABIN296462)

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reaktivität: Cat

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser CCL5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut), Functional Studies (Func)

Produktdetails anti-CCL5 Antikörper

Spezifität: Selected for its ability to neutralize feline RANTES bioactivity.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rfeRANTES.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Neutralization. Neutralization of Feline CCL5 Bioactivity: The exact concentration of antibody required to neutralize rfeRANTES activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-feline RANTES antibody was determined to be approximately 0.5-2.5 μg/mL in the presence of 80 ng/mL of rfeRANTES, using transfected BaF/3 cells in a chemotaxis assay. The specific conditions are described in the figure legends. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 1 ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 0.5 ng/well. In this format, this antibody shows less than 5 % cross-reactivity with rcrRANTES, rhRANTES, and rmRANTES.

Kommentare:

Target Species of Antibody: Cat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu CCL5

Target: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Andere Bezeichnung: CCL5 / RANTES

Hintergrund: Name/Gene ID: CCL5
Family: Intercrine

Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228

Gen-ID: 6352

UniProt: P13501

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration