DLL1 Antikörper (Extracellular Domain)

Produktnummer ABIN295726

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch DLL1 in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für DLL1 Antikörper (Extracellular Domain) (ABIN295726)

Target: DLL1 (delta-Like 1 (DLL1))

Reaktivität: Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser DLL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-DLL1 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognize rat DLL1. Species sequence homology: mouse (97 % ), human (91 %), over aa18-537.

Aufreinigung: Affinity purified

Sterilität: 0.2 μm filtered

Immunogen: NS0-derived rrDLL1 extracellular domain.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 μg/mL Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL Detection limit: 0.3 ng/well.~10 % cross-reactivity with rhDLL-1 and >1 % cross reactivity with rrNotch-1.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 40-50 % glycerol, PBS.

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Andere Bezeichnung: DLL1

Hintergrund: Name/Gene ID: DLL1

Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa

Gen-ID: 28514

UniProt: O00548

Pathways: Notch Signalweg, Stem Cell Maintenance