DLL1 Antikörper (Extracellular Domain)

Produktnummer ABIN295726

Dieser Schaf Polyklonal Antikörper detektiert spezifisch DLL1 in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für DLL1 Antikörper (Extracellular Domain) (ABIN295726)

Target: DLL1 (delta-Like 1 (DLL1))

Reaktivität: Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser DLL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-DLL1 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognize rat DLL1. Species sequence homology: mouse (97 % ), human (91 %), over aa18-537.

Aufreinigung: Affinity purified

Sterilität: 0.2 μm filtered

Immunogen: NS0-derived rrDLL1 extracellular domain.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Western Blot: 0.1-0.2 μg/mL Detection limit for recombinant rat DLL1 is 5 ng/lane under non-reducing and 25 ng/lane under reducing conditions. Immunohistochemistry: 10 μg/mL Detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 min at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL Detection limit: 0.3 ng/well.~10 % cross-reactivity with rhDLL-1 and >1 % cross reactivity with rrNotch-1.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 40-50 % glycerol, PBS.

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Andere Bezeichnung: DLL1

Hintergrund: Name/Gene ID: DLL1

Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa

Gen-ID: 28514

UniProt: O00548

Pathways: Notch Signalweg, Stem Cell Maintenance