Plexin A3 Antikörper (AA 34-150)

Produktnummer ABIN295494

Dieses Anti-Plexin A3-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Plexin A3 in ELISA, IHC und WB. Geeignet für Maus.

Kurzübersicht für Plexin A3 Antikörper (AA 34-150) (ABIN295494)

Target: Plexin A3 (PLXNA3)

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Plexin A3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails anti-Plexin A3 Antikörper

Bindungsspezifität: AA 34-150

Spezifität: Selected for its ability to recognize mouse Plexin A3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5 % cross-reactivity with rmPlexin A1, rmPlexin A2, rhPlexin B1, rhPlexin C1, and rhPlexin D1.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived recombinant mouse Plexin A3 (aa34-150).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Plexin A3. The detection limit for rmPlexin A3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Plexin A3 in cryosections of E13 mouse embryos. Tissue was fixed overnight in 4 % PFA+14 %picric acid in PBS at 4? C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at room temperature. After blocking, sections were incubated with primary antibody for 3 hours at room temperature followed by NorthernLights 557 donkey anti-goat lgG at room temperature for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Plexin A3. The detection limit for rmPlexin A3 is approximately 1.0 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu Plexin A3

Target: Plexin A3 (PLXNA3)

Andere Bezeichnung: PLXNA3 / Plexin A3

Hintergrund: Name/Gene ID: PLXNA3

Synonyms: PLXNA3, 6.3, HSSEXGENE, Plexin-A3, SEX, Plexin A3, Plexin-4, PLXN3, PLXN4, XAP-6, Semaphorin receptor SEX

Gen-ID: 55558

UniProt: P51805

Pathways: Regulation of Cell Size