MEK1 + MEK2 Antikörper (pSer217, Ser221)
Kurzübersicht für MEK1 + MEK2 Antikörper (pSer217, Ser221) (ABIN294413)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pSer217, Ser221
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Spezifität
- Detects endogenous levels of MEK1/2 only when activated by phosphorylation at Ser217/221. Does not cross-react with related kinases including activated SEK (MKK4), MKK3 or MKK6. It will also react with MEK1/2 singly phosphorylated at Ser217, but not with MEK1/2 singly phosphorylated at Ser221. Species cross-reactivity: Human, mouse and rat.
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Aufreinigung
- Protein A purified
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Immunogen
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Synthetic phosphopeptide corresponding to residues around Ser217/221 of human MEK1/2 (KLH coupled). MW: 45kD.
Type of Immunogen: Synthetic phospho-peptide -
Isotyp
- IgG
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Applikationshinweise
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Approved: IP (1:50), WB (1:1000)
Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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Konservierungsmittel
- Without preservative
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Handhabung
- Avoid repeat freeze-thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- MEK1 + MEK2
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Andere Bezeichnung
- MEK1 + MEK2
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Hintergrund
- This antibody detects: MEK1 + MEK2
Target
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