UTF1 Antikörper (AA 44-153)

Produktnummer ABIN293228

Der Ziege Polyklonal Anti-UTF1-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, UTF1 in Proben von Human zu detektieren.

Kurzübersicht für UTF1 Antikörper (AA 44-153) (ABIN293228)

Target: UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UTF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-UTF1 Antikörper

Bindungsspezifität: AA 44-153

Spezifität: Selected for its ability to recognize UTF1 in the applications listed.

Aufreinigung: Affinity purified

Sterilität: 0.2 μm filtered

Immunogen: E. coli-derived rhUTF1 (aa44-153).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 10 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. Immunocytochemistry: This antibody can be used at a concentration of 10 μg/mL to detect UTF1 in mouse D3 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Northern Lights 557-coupled anti-donkey IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect UTF1. The detection limit for rhUTF1 is approximately 1.0 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile 40-50 % glycerol, PBS

Konzentration: Lot specific

Buffer: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5 % trehalose.

Handhabung: Aviod frequent freeze thaws.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Details zu UTF1

Target: UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1 (UTF1))

Andere Bezeichnung: UTF1

Hintergrund: Name/Gene ID: UTF1

Synonyms: UTF1

Gen-ID: 8433

UniProt: Q5T230