PAX8 Antikörper (AA 2-14)

Produktnummer ABIN292477

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PAX8 in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund, Kaninchen und Affe.

Kurzübersicht für PAX8 Antikörper (AA 2-14) (ABIN292477)

Target: PAX8 (Paired Box 8 (PAX8))

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PAX8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PAX8 Antikörper

Bindungsspezifität: AA 2-14

Spezifität: Human PAX8. This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1)

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Elephant, Rabbit, Opossum, Platypus (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide PHNSIRSGHGGLN-C from the N-terminus of human PAX8 (NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Elephant, Rabbit, Opossum, Platypus (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Andere Bezeichnung: PAX8

Hintergrund: Name/Gene ID: PAX8

Synonyms: PAX8, Paired box 8, Paired domain gene 8, Paired box gene 8, Paired box protein Pax-8

Gen-ID: 7849

NCBI Accession: NP_003457, NP_039245, NP_039246, NP_039247, NP_054698

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation