HAVCR1 Antikörper (AA 200-300)

Produktnummer ABIN292372

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch HAVCR1 in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für HAVCR1 Antikörper (AA 200-300) (ABIN292372)

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HAVCR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HAVCR1 Antikörper

Bindungsspezifität: AA 200-300

Spezifität: The amino acid sequence (SPLYSYTTDGNDTVT) used as immunogen for this is 100 % homologous in human, 93 % homologous in vervet/green monkey and 80 % homologous in chimp. Isoforms of different molecular weights have been described for TIM-1. The sequence used for immunogen is conserved between TIM-1 (364aa, NP_036338) and TIM-1 isoform CRA_a (194aa, EAW61615.1). Therefore, the molecular weight observed in western blots may vary depending on the forms present.

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Monkey (93%) Dog (80%).

Aufreinigung: Protein G purified

Immunogen: A synthetic peptide (SPLYSYTTDGNDTVT) of human HAVCR1/TIM-1protein were used as immunogen. This sequence corresponds to amino acids (aa) 248-262 of NP_036338 and aa 84-98 of EAW61615.1. Isoforms of HAVCR1/TIM-1 have been described. For example, NP_036338 is 364 aa and EAW61615.1 is 194 aa. EAW61615.1 is referred to as isoform CRA_a. Percent identity by BLAST analysis: Human, Gorilla (100%), Monkey (93%), Dog (80%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 - 5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Details zu HAVCR1

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Andere Bezeichnung: HAVCR1 / KIM-1

Substanzklasse: Virus

Hintergrund: Name/Gene ID: HAVCR1

Synonyms: HAVCR1, HAVCR-1, KIM-1, Kidney injury molecule 1, KIM1, T-cell membrane protein 1, TIM1, TIMD1, TIM-1, HAVCR, TIMD-1

Gen-ID: 26762

NCBI Accession: NP_036338

UniProt: Q96D42