Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

HES4 Antikörper (AA 94-221)

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch HES4 in WB, ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN291868

Kurzübersicht für HES4 Antikörper (AA 94-221) (ABIN291868)

Target

Alle HES4 Antikörper anzeigen
HES4 (Hairy and Enhancer of Split 4 (HES4))

Reaktivität

  • 9
  • 2
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 5
  • 2
  • 1
  • 1
Schaf

Klonalität

  • 9
Polyklonal

Konjugat

  • 8
  • 1
Dieser HES4 Antikörper ist unkonjugiert

Applikation

  • 8
  • 4
  • 1
  • 1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 2
    • 2
    • 1
    • 1
    • 1
    AA 94-221

    Spezifität

    Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.

    Aufreinigung

    Affinity purified

    Immunogen

    E. coli-derived rhHES-4 (aa94-221).

    Isotyp

    IgG
  • Applikationshinweise

    Approved: ELISA, ICC, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    deionized water

    Konzentration

    Lot specific

    Buffer

    Lyophilized.
  • Target

    HES4 (Hairy and Enhancer of Split 4 (HES4))

    Andere Bezeichnung

    HES4

    Hintergrund

    Name/Gene ID: HES4

    Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4

    Gen-ID

    57801

    UniProt

    Q9HCC6
Sie sind hier:
Chat with us!