HSP70 Antikörper

Produktnummer ABIN291550

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HSP70 in WB, IHC, IF, IP, FACS, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh) und Affe.

Kurzübersicht für HSP70 Antikörper (ABIN291550)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reaktivität: Human, Rind (Kuh), Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-HSP70 Antikörper

Spezifität: Recognizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. Species cross-reactivity: Human, monkey and bovine.

Aufreinigung: Protein A purified

Immunogen: HSP70 protein from HeLa cells. Cellular Localization: Nuclear and cytoplasmic.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IF, IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native & denatured): 2 μg antibody/mg protein lysate using Protein A. Western Blot: 0.5-1 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen & formalin/paraffin): 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: BT474 or HeLa cells, breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Andere Bezeichnung: HSP70 / Heat Shock Protein 70

Hintergrund: Name/Gene ID: HSP70

Synonyms: HSP70