Latexin Antikörper (AA 2-222)

Produktnummer ABIN291079

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch Latexin in WB, ELISA, IP und Neut. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Latexin Antikörper (AA 2-222) (ABIN291079)

Target: Latexin (LXN)

Reaktivität: Maus

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Latexin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Produktdetails anti-Latexin Antikörper

Bindungsspezifität: AA 2-222

Spezifität: Selected for its ability to recognize mouse Latexin in direct ELISAs and Western Blots. In these formats, this antibody shows ~40 % cross-reactivity with rhLatexin.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rmLatexin (aa2-222).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IP, Neut, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 5 ng/lane and 0.5 ng/lane under non-reducing and reducing conditions, respectively. Immunoprecipitation: This antibody has been used to immunoprecipitate rmLatexin from conditioned media of transfected NS0 cells. Neutralization: This antibody has been used to neutralize the inhibitory activity of rmLatexin against rmCPA. The antibody was preincubated with the inhibitor at different molar ratios at room temperature for 30 minutes. The resulting mixtures were then incubated with the enzyme at room temperature for 60 minutes. The enzyme was assayed with a peptide substrate. 50 % of the proteolytic activity was restored by the antibody at 4.4 μg/mL (IC50) under conditions in which 0.1 μg/mL of the inhibitor, 0.1 μg/mL of the enzyme, and 100 M of the substrate were present. Considering the molecular masses of the inhibitor (26kD) and the antibody (150kD), IC50 was achieved at approximately 8:1 Molar ratio of the antibody to the inhibitor. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Latexin. The detection limit for rmLatexin is approximately 0.3 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu Latexin

Target: Latexin (LXN)

Andere Bezeichnung: Latexin / MUM

Hintergrund: Name/Gene ID: LXN

Synonyms: LXN, ECI, MUM, Protein MUM, TCI, Latexin

Gen-ID: 56925

UniProt: Q9BS40