AIRE Antikörper (AA 534-545)

Produktnummer ABIN291034

Dieser Ziege Polyklonal Antikörper detektiert spezifisch AIRE in ELISA, IHC, IHC (p) und RIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für AIRE Antikörper (AA 534-545) (ABIN291034)

Target: AIRE (Autoimmune Regulator (AIRE))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AIRE Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Produktdetails anti-AIRE Antikörper

Bindungsspezifität: AA 534-545

Spezifität: Human AIRE. This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QSMARPAAPFPS from the C-terminus of human AIRE (NP_000374.1, NP_000649.1). Percent identity by BLAST analysis: Human, Gorilla (100%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), RIA (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Andere Bezeichnung: AIRE

Hintergrund: Name/Gene ID: AIRE

Synonyms: AIRE, APSI, AIRE1, Autoimmune regulator, APECED, APS1, APECED protein, PGA1

Gen-ID: 326

NCBI Accession: NP_000374, NP_000649

UniProt: O43918

Pathways: Chromatin Binding