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UCP1 Antikörper (AA 179-296)

UCP1 Reaktivität: Maus WB, IHC, IP, IF/ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2905048
  • Target Alle UCP1 Antikörper anzeigen
    UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))
    Bindungsspezifität
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 179-296
    Reaktivität
    • 28
    • 19
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 35
    • 4
    • 1
    Kaninchen
    Klonalität
    • 37
    • 3
    Polyklonal
    Konjugat
    • 20
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser UCP1 Antikörper ist unkonjugiert
    Applikation
    • 29
    • 17
    • 14
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)
    Aufreinigung
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    UCP1 (Pro179-Leu296)
    Top Product
    Discover our top product UCP1 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #103357 (Western Blotting)
    'Independent Validation' Siegel
    by
    Mivechi lab, Georgia Cancer Center, Augusta University
    No.
    #103357
    Datum
    03.10.2018
    Antigen
    UCP1
    Chargennummer
    AB1808U769
    Validierte Anwendung
    Western Blotting
    Positivkontrolle

    Cold exposed mouse brown adipose tissue (BAT)

    Negativkontrolle

    Mouse liver tissue

    Bewertung

    Passed. ABIN3056733 can detect mouse UCP1 protein in immunoblotting analysis.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN3056733
    Sekundärantikörper
    donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152)
    Full Protocol
    • Homogenize mouse brown fat tissue in 2ml mitochondria isolation buffer (70mM sucrose, 210mM mannitol, 5mM HEPES, 1mM EGTA, pH7.2, and 0.5% BSA).
    • Centrifuge the homogenates for 10min at 800g at 4°C.
    • Centrifuge the supernatant again at 8000g for 10min at 4°C.
    • Lyse the resulting mitochondria fraction in RIPA buffer containing protease inhibitor cocktail (Pierce Protease Inhibitor, A32965, lot TA2492391).
    • Determined protein concentration with Pierce BCA Protein Assay Kit (Pierce, 23225, lot OA180049).
    • Separate 50µg of total protein per lane on a denaturing 12% SDS-PAGE gel (Bio-Rad, 1610156, lot 64144809) in a Mini Protean Tetra Cell for 2h at 80V.
    • Transfer proteins onto Immobilon-P PVDF Membrane (Millipore, IPVH00010) using Mini Trans Blot Cell system (Bio-Rad, 1703930).
    • Block membrane with 5% milk in PBS-T for 1h at RT.
    • Incubate membrane with primary rabbit anti-UCP1 antibody (antibodies-online, ABIN3056733, lot AB1808U769) diluted to 1µg/ml ON at 4°C.
    • Wash membrane 3x for 10min with PBS-T.
    • Incubate membrane with secondary donkey anti-rabbit HRP-conjugated antibody (Jackson Immuno Research, 711-035-152) for 1h at RT.
    • Wash membrane 3x for 10min with PBS-T.
    • Incubate membrane Pierce ECL Western Blotting Substrate working solution (Thermo Scientific, 1859701, lot TB263310) for 1min at RT. Expose membrane to x-ray film for different amount of time.
    Anmerkungen

    The UCP1 antibody ABIN3056733 reveals a specific protein band in positive control samples (cold exposed mouse brown fat tissue), but not in negative control samples (liver tissue).

  • Format
    Liquid
    Konzentration
    500 μg/mL
    Buffer
    0.01M PBS,  pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
  • Target
    UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))
    Andere Bezeichnung
    Uncoupling Protein 1, Mitochondrial (UCP1 Produkte)
    Synonyme
    ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1 antikoerper, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1 antikoerper, ATPUMP1 antikoerper, ATUCP1 antikoerper, UCP antikoerper, UCP1 antikoerper, UNCOUPLING PROTEIN 1 antikoerper, plant uncoupling mitochondrial protein 1 antikoerper, ucp antikoerper, slc25a7 antikoerper, SLC25A7 antikoerper, AI385626 antikoerper, Slc25a7 antikoerper, Ucp antikoerper, Ucpa antikoerper, Uncp antikoerper, plant uncoupling mitochondrial protein 1 antikoerper, uncoupling protein 1 antikoerper, uncoupling protein 1 L homeolog antikoerper, uncoupling protein 1 (mitochondrial, proton carrier) antikoerper, PUMP1 antikoerper, UCP1 antikoerper, ucp1.L antikoerper, ucp1 antikoerper, Ucp1 antikoerper
    UniProt
    P12242
    Pathways
    Proton Transport, Brown Fat Cell Differentiation
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