Tumor Protein p73 Antikörper (AA 75-125)

Produktnummer ABIN290398

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Tumor Protein p73 in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hund, Affe und Hamster.

Kurzübersicht für Tumor Protein p73 Antikörper (AA 75-125) (ABIN290398)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Affe, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tumor Protein p73 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Tumor Protein p73 Antikörper

Bindungsspezifität: AA 75-125

Spezifität: This peptide sequence is 100 % conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Researchers are encourage to use the the immunogen sequence information and available Bioinformatics data bases to obtain additional information regarding the amino acid sizes associated with various p73 isoforms.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine (100%) Opossum, Chicken, Xenopus (90%) Bat, Rabbit, Turkey, Stickleback, Medaka, Pufferfish, Zebrafish, Nectri, Fusarium (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: A synthetic peptide C-THSPYAQPSS corresponding to amino acids 95-104 of human p73, GenBank EAW71468.1. This peptide sequence is 100% conserved across multiple p73 isoforms and between rat, mouse, dog and bovine p73. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine (100%), Opossum, Chicken, Xenopus (90%), Bat, Rabbit, Turkey, Stickleback, Medaka, Pufferfish, Zebrafish, Nectri, Fusarium (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Andere Bezeichnung: TP73 / p73

Hintergrund: Name/Gene ID: TP73

Synonyms: TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

Gen-ID: 7161

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus