Alkaline Phosphatase Antikörper (HRP)
Kurzübersicht für Alkaline Phosphatase Antikörper (HRP) (ABIN288216)
Target
Alle Alkaline Phosphatase (ALP) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Kreuzreaktivität (Details)
- No cross reactivity against mammalian forms of alkaline Phosphatase.
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Aufreinigung
- Affinity chromatography purified
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Immunogen
- Alkaline phosphatase antibody (HRP) was raised in rabbit using alkaline phosphatase isolated from E.coli as the immunogen.
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Applikationshinweise
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ELISA: 1:4,000-1:20,000, WB: 1:500-1:2,000
Optimal conditions should be determined by the investigator. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Konzentration
- Lot specific
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Buffer
- Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add Sodium azide. Immunoglobulin and protease free.
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Handhabung
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Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use. -
Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- Alkaline Phosphatase (ALP)
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Andere Bezeichnung
- Alkaline Phosphatase
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Hintergrund
- Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Target
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